Collagen Coated 6 Well Plate

We use 2 5mg ml collagen from rat tail sigma stock solution in 0 2 sterile acetic.

Collagen coated 6 well plate.

Corning biocoat collagen i 6 well clear multiwell plate is comprised of clear tc treated polystyrene with a uniform application of rat tail collagen type i. Gibco collagen i rat tail is now available in pre coated tissue culture plates to help save time at the bench. The grade of jellagen jellyfish collagen used to coat this. Ready to use room temperature storage.

Collagen i found in most tissues and organs is most plentiful in dermis tendon and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. 266 µl of naoh 0 1 m. Combine 5 6 ml of 10x pbs with 194 4 ml of 1x dpbs.

To coat culture wells we dilute 20μl of collagen stock in 1ml of 30 ethanol per well 6 well plates and. Flat bottom 5 pack 50 case 389 83 per case. We offer collagen i rat tail in liquid form and on precoated tissue culture plates and collagen i bovine in liquid form for 3d applications and as coating solution. In vitro use of collagen can exert effects on the adherence morphology growth migration and differentiation of a variety of cell types.

Under the culture hood prepare a collagen coating solution 300 μg ml by combining the following into a 50 ml centrifuge tube or similar sterile container in order. Procedure for one 6 well plate or 12 well plate under the culture hood prepare 200 ml of 1 25x dpbs. Designed to meet a number of different applications our high quality consistent collagen i coated plates come in 3 formats 96 well 24 well and 6 well plates.